T Cell Expansion

Rapamycin Inhibits Expansion of Cord Blood Derived NK and T Cell

Background: The mammalian target of rapamycin (mTOR) is important in hematopoiesis. Despite the central role of mTOR in regulating the differentiation of immune cells, the effect of mTOR function on cord blood mononuclear cells is yet to be defined. Objectives: To evaluate the effect of mTOR inhibition, using rapamycin on the proliferation and apoptosis of cord blood mononuclear cells, as well ...

Antigen-specific T-T interactions regulate CD4 T-cell expansion.

The regulation of CD4 T-cell numbers during an immune response should take account of the amount of antigen (Ag), the initial frequency of Ag-specific T cells, the mix of naive versus experienced cells, and (ideally) the diversity of the repertoire. Here we describe a novel mechanism of T-cell regulation that potentially deals with all of these parameters. We found that CD4 T cells establish a ...

CD40 activation boosts T cell immunity in vivo by enhancing T cell clonal expansion and delaying peripheral T cell deletion.

In this report we show that activation of APC with an agonist anti-CD40 mAb profoundly alters the behavior of CD4 T cells in vivo. Stimulation of mice with anti-CD40 2 days before, but not 1 day after, administration of superantigen (SAg) enhanced CD4 and CD8 T cell clonal expansion by approximately threefold. Further, CD40 activation also delayed peripheral T cell deletion after activation. Dy...

The impact of T cell percentage at CTL019 T cell expansion process initiation on post-harvest T cell composition.

Results The current CTL019 process targets 450e9 total nucleated cells (TNC) at seeding of the static culture bags prior to activation and transduction (Kalos M et al. 2011; Maude et al. 2014). However, less is known on the impact of a range of T cell numbers within the TNC population on, transduction efficiency and expansion. Healthy donor T cells cultures were evaluated in the range of the st...

Preferential T cell Expansion by Artificial Antigen Presenting Cells Expressing 4-1BBL Alone or in Combination with CD80 or CD86